Intsholongwane kaZika

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukubona ngokusemgangathweni i-Zika virus nucleic acid kwiisampulu zeserum yezigulane ezirhanelwa ukuba zintsholongwane kaZika kwi-vitro.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Isatifikethi

CE

I-Epidemiology

Intsholongwane yeZika yeyohlobo lweFlaviviridae, yintsholongwane yeRNA enemisonto enye enemisonto enye enobubanzi obuyi-40-70nm.Inemvulophu, iqulethe i-nucleotides ye-10794, kunye ne-encodes 3419 amino acids.Ngokutsho kwe-genotype, ihlukaniswe ngohlobo lwase-Afrika kunye nohlobo lwase-Asia.Isifo sentsholongwane yeZika sisifo esosulelayo esizinciphisayo esibangelwa yintsholongwane yeZika, esasazeka ngokulunywa ziingcongconi ze-Aedes aegypti.Iimpawu zeklinikhi ikakhulu ngumkhuhlane, irhashalala, i-arthralgia okanye i-conjunctivitis, kwaye ayifane ibulale.Ngokutsho koMbutho wezeMpilo weHlabathi, i-microcephaly ye-neonatal kunye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) inokudibaniswa nosulelo lwe-Zika virus.

Umjelo

FAM Zika virus nucleic acid
ROX

Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino ≤30℃ kwaye ikhuselwe ekukhanyeni
Beka ubomi kwishelufa Iinyanga ezili-12
Uhlobo loMfanekiso i-serum entsha
Ct ≤38
CV ≤5.0%
LoD 500 ng/μL
Ukuchaza ngokuthe ngqo Iziphumo zovavanyo ezifunyenwe yile kit aziyi kuchaphazeleka nge-hemoglobin (<800g / L), i-bilirubin (<700μmol / L), kunye ne-lipids yegazi / triglycerides (<7mmol / L) egazini.
Izixhobo Ezisebenzayo Iinkqubo zePCR ze-ABI 7500

ABI 7500 Fast Real-Time PCR Systems

QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, iBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani

Ukuhamba komsebenzi

Ukhetho loku-1.

I-QIAamp Viral RNA Mini Kit(52904), iNucleic Acid Extraction okanye iReagent yokuCoca(YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ukutsalwakufuneka itsalwe ngokwemiyalelo yokutsalwa, kwaye umthamo wokutsalwa ocetyiswayo yi-140 μL kunye nomthamo we-elution ocetyiswayo yi-60 μL.

Ukhetho lwesi-2.

IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ukutsalwa kufuneka kutsalwe ngokwemiyalelo.Umthamo wesampulu yokutsalwa ngama-200 μL, kwaye umthamo we-elution ocetyiswayo yi-80μL.


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