I-Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Isothermal Amplification)

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-Neisseria gonorrhoeae nucleic acid kwiisampuli ze-genitourinary tract in vitro.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-UR026-Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Isatifikethi

I-Epidemiology

I-Gonorrhea sisifo se-classical transmitted infections esibangelwa ukusuleleka nge-Neisseria gonorrhoeae (NG), ebonakalisa ngokubonakalayo njengokuvuvukala kwe-purulent ye-mucous yenkqubo ye-genitourinary.Ngo-2012, uMbutho wezeMpilo weHlabathi (i-WHO) waqikelela ukuba kukho iimeko ezizigidi ezingama-78 kubantu abadala ehlabathini lonke.I-Neisseria gonorrhoeae ihlasela inkqubo ye-genitourinary kwaye izala, ibangela urethritis kwindoda kunye ne-urethritis kunye ne-cervicitis kwabasetyhini.Ukuba ayinyangwa ngokupheleleyo, inokusasazeka kwinkqubo yokuzala.Usana olungekazalwa lungosuleleka kumjelo wokubeleka okukhokelela kwi-neonatal gonorrhea acute conjunctivitis.Abantu abanalo ukhuselo lwendalo lwe-Neisseria gonorrhoeae, kwaye bonke banokuchaphazeleka.Ukhuselo lomzimba emva kokugula alunamandla kwaye alunakukuthintela ukosuleleka kwakhona.

Umjelo

FAM	NG nucleic acid
CY5	Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo loMfanekiso	Umchamo wamadoda, i-urethral swab yamadoda, i-swab yomlomo wesibeleko yabasetyhini
Tt	≤28
CV	≤5.0%
LoD	50pcs/mL
Ukuchaza ngokuthe ngqo	Akukho cross-reactivity kunye nezinye izifo ze-genitourinary usulelo ezifana nomngcipheko ophezulu HPV uhlobo 16, papillomavirus uhlobo 18, herpes simplex virus uhlobo 2, treponema pallidum, M.hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia Candida alpha, Garbicans , Trichomonas vaginalis, L.crispatus, adenovirus, cytomegalovirus, Group B Streptococcus, HIV virus, L.casei, kunye neDNA genome yabantu.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha elililo (Hongshi Medical Technology Co., Ltd.), iLightCycler®480 inkqubo yePCR yexesha elililo, iFluorescence yeXesha eliQoqo kwiNkqubo yokuFumana ubushushu obuLula i-Amp HWTS1600

Ukuhamba komsebenzi

1. ISampuli yoQokelelo
① I-swab yomlomo wesibeleko yowesifazane: hlamba i-extracts yangaphandle yomlomo wesibeleko, emva koko ufake i-swab yebhola yomqhaphu engenazintsholongwane emlonyeni wesibeleko, ujikelezise i-swab imizuzwana emi-5.Beka i-swab kwityhubhu yesampulu engenazintsholongwane, vala umnqwazi wetyhubhu, kwaye uyitywine ukuze uyivavanye.
② I-swab ye-urethral yamadoda: sebenzisa i-swab ukufaka kwi-urethra ye-2-4cm, kwaye ujije i-swab ukuqokelela imfihlo.Beka i-swab kwityhubhu yesampulu engenazintsholongwane, vala umnqwazi wetyhubhu, kwaye uyitywine ukuze uyivavanye.
③ Umchamo wendoda: Thatha umchamo wokuqala ekuseni, okanye uthathe i-4.5mL yomchamo wokuqala emva kwexesha elide ngaphandle kokuchama, xuba kunye ne-0.5mL yesisombululo sokugcina umchamo.
2. Inkqubo yeSampuli
Yongeza i-1-3mL ye-saline engenazintsholongwane kwityhubhu engenazintsholongwane equlethe isampulu yeswab, hlunguza ngokupheleleyo kwaye udibanise kakuhle, cudisa umqhaphu womile, kwaye uwulahle.

Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test yeSampuli yokuKhupha iReagent (HWTS-3005-8).
Ukhetho lwesi-2.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ngaphambili: Ureaplasma Urealyticum Nucleic Acid Detection Kit (Isothermal Amplification)

Okulandelayo: I-Hepatitis B Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Bhala umyalezo wakho apha kwaye uwuthumele kuthi