KRAS 8 Utshintsho

Inkcazelo emfutshane:

Le khithi yenzelwe ukufumanisa umgangatho we-in vitro we-8 utshintsho kwi-codons 12 kunye ne-13 ye-K-ras gene kwi-DNA ekhutshiweyo kumacandelo e-pathological yabantu afakwe kwiparafini.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-TM014-KRAS 8 Izixhobo zokuFumana iiNguqu (i-Fluorescence PCR)

I-HWTS-TM011-eyomiswe ngumkhenkce iKRAS 8 iMutations Detection Kit(Fluorescence PCR)

Isatifikethi

CE

I-Epidemiology

Utshintsho lwamanqaku kwi-gene ye-KRAS lufunyenwe kwinani leentlobo zethumba lomntu, malunga ne-17% ~ 25% yezinga lokuguqulwa kwe-tumor, i-15% ~ 30% yezinga lokuguqula kwizigulane zomhlaza wemiphunga, i-20% ~ 50% yezinga lokuguqulwa komhlaza we-colorectal. izigulane.Ngenxa yokuba iprotheyini ye-P21 efakwe kwi-K-ras gene ibekwe ezantsi kwendlela yokubonisa i-EGFR, emva kokuguqulwa kwe-K-ras ye-gene, indlela yokubonisa i-downstream isoloko ivuliwe kwaye ayichatshazelwa ngamachiza ajoliswe kuyo phezulu kwi-EGFR, okubangelwa ukuqhubeka. ukwanda okuyingozi kweeseli.Utshintsho kumfuza we-K-ras ngokubanzi lunika ukuxhathisa kwi-EGFR i-tyrosine kinase inhibitors kwizigulana ezinomhlaza wemiphunga kunye nokuchasana ne-anti-EGFR antibody amachiza kwizigulana ezinomhlaza we-colorectal.Ngo-2008, i-National Comprehensive Cancer Network (NCCN) yakhupha isikhokelo sonyango lomhlaza womhlaza, owabonisa ukuba iindawo eziguqukayo ezibangela ukuba i-K-ras isebenze ikakhulu ibekwe kwi-codons 12 kunye ne-13 ye-exon 2, kwaye kucetyiswa ukuba zonke izigulana ezinomhlaza ophambili we-metastatic colorectal zinokuvavanyelwa utshintsho lwe-K-ras phambi konyango.Ke ngoko, ukufunyaniswa okukhawulezileyo nokuchanekileyo kokuguqulwa kofuzo lwe-K-ras kubaluleke kakhulu kwisikhokelo samayeza ezonyango.Le khithi isebenzisa i-DNA njengesampulu yokufumanisa ukubonelela ngovavanyo olusemgangathweni lwesimo soguqulo, olunokunceda oogqirha ekuhloleni umhlaza we-colorectal, umhlaza wemiphunga kunye nezinye izigulana zethumba ezixhamla kumachiza ekujoliswe kuwo.Iziphumo zovavanyo lwekhithi zezereferensi yeklinikhi kuphela kwaye akufanele zisetyenziswe njengesiseko sodwa sonyango lwezigulane.Oogqirha kufuneka benze izigwebo ezibanzi kwiziphumo zovavanyo ngokusekelwe kwizinto ezifana nemeko yesigulane, izibonakaliso zamachiza, impendulo yonyango kunye nezinye izikhombisi zovavanyo lwebhubhoratri.

Iiparamitha zobuGcisa

Ugcino Ulwelo: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Beka ubomi kwishelufa Ulwelo: iinyanga ezili-9;I-Lyophilized: iinyanga ezili-12
Uhlobo loMfanekiso iparafini-embedded pathological tissue okanye icandelo iqulethe iiseli ithumba
CV ≤5.0%
LoD I-K-ras iRection Buffer A kunye ne-K-ras Reaction Buffer B inokubona ngokuzinzileyo i-1% yesantya soguqulo phantsi kwe-3ng/μL yohlobo lwasendle lwangasemva
Izixhobo Ezisebenzayo Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7300 Iinkqubo zePCR zeXesha langempela

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

I-LightCycler® 480 inkqubo ye-PCR yeXesha langempela

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene

Ukuhamba komsebenzi

Kuyacetyiswa ukuba kusetyenziswe iQIAGEN's QIAamp DNA FFPE Tissue Kit (56404) kunye neParaffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yiTiangen Biotech(Beijing) Co., Ltd.


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