I-Methylated yomntu NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Inkcazelo emfutshane:

Ikiti ihloselwe ukufunyanwa kwe-in vitro qualitative ye-methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 izakhi zofuzo kwiiseli ze-intestinal exfoliated kwiisampuli zesitulo somntu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Ikhithi yokubona iGene (Fluorescence PCR)

Isatifikethi

CE

I-Epidemiology

Kubantu abadala, ngaphezu kwe-10 8 iiseli ze-epithelial zamathumbu ziwela eludongeni lwamathumbu yonke imihla, kwaye zikhutshwe kunye nendle nge-peristalsis enkulu yamathumbu.Ngenxa iiseli ithumba kusenokwenzeka ukuba bawe kwi iphecana wamathumbu ukwanda engaqhelekanga, isitulo izigulana ithumba emathunjini iqulathe iiseli ezininzi ezigulayo kunye namacandelo iiseli engaqhelekanga, nto leyo isiseko eziphathekayo ukubhaqa isitulo esizinzileyo.Uphononongo lufumene ukuba ukuguqulwa kwe-methylation yabakhuthazi bemfuza sisiganeko sokuqala kwi-tumorigenesis, kwaye izinto zofuzo ezifunyenwe kwiisampulu zesitulo sezigulane ezinomhlaza we-colorectal zingabonakalisa ubukho bomhlaza emathunjini ngaphambili.

I-NDRG4, eyaziwa ngokuba yi-SMAP-8 kunye ne-BDM1, ingomnye wamalungu amane e-NDRG gene family (NDRG1-4), ebonakaliswe ukuba idibene nokwanda kweeseli, ukuhlukana, ukuphuhliswa kunye noxinzelelo.Kuqinisekisiwe ukuba i-NDRG4 methylation yinto enokubakho ye-biomarker yokubhaqwa okungahlaseli komhlaza we-colorectal kwiisampuli zesitulo.

I-SEPT9 lilungu lentsapho ye-Septin yemfuza, equkethe ubuncinane i-13 yofuzo efaka i-domain ye-GTPase egciniweyo enokuthi ibophe iiprotheni ezinxulumene ne-cytoskeleton, kwaye idibene nokwahlulwa kweseli kunye ne-tumorigenesis.Uphononongo lufumene ukuba umxholo we-methylated Septin9 wofuzo wonyuswa ngokwesiqhelo kwiisampulu zesitulo kwizigulana ezinomhlaza obala.

Iiprotheyini ezinxulumene ne-frizzled-related frizzled (sFRPs) ziiprotheni ezinyibilikayo eziyiklasi ye-Wnt pathway antagonists ngenxa ye-homology ephezulu yesakhiwo kwi-frizzled (Fz) receptor ye-Wnt signaling.Ukungasebenzi kwemfuza ye-SFRP kubangela ukusetyenziswa okungalawulwayo kwe-Wnt yokubonisa ehambelana nomhlaza wesisu.Okwangoku, i-SFRP2 methylation kwi-stool inokusetyenziswa njenge-biomarker engahlaseliyo yokuxilongwa komhlaza we-colorectal.

I-BMP3 lilungu le-TGF-B superfamily kwaye ngaloo ndlela idlala indima ebalulekileyo kuphuhliso lwe-embryonic ngokukhuthaza kunye nokubumba ukwakheka kwamathambo kwangethuba.I-BMP3 yi-hypermethylated kumhlaza webala kwaye inokusetyenziswa njengephawu elibalulekileyo lethumba.

I-SDC2 yiseli yomphezulu we-heparan sulfate proteoglycan ebandakanyeka kulawulo lweenkqubo ezininzi ze-physiological and pathological.Ukucutshungulwa ngokomzimba kubandakanya ukwanda kweeseli, ukwahlula, ukunamathela, umbutho we-cytoskeletal, ukufuduka, ukuphulukiswa kwesilonda, ukunxibelelana kwe-cell-matrix, i-angiogenesis;Iinkqubo ze-Pathological ziquka ukuvuvukala kunye nomhlaza.Inqanaba le-methylation ye-SDC2 yofuzo kwizicubu zomhlaza we-colorectal laliphezulu kakhulu kunelo lihlala kwizicubu eziqhelekileyo.

Umjelo

Isithinteli sokuphendula A

VIC/HEX Ufuzo lwe-methylated NDRG4
ROX Ufuzo lwe-methylated SEPT9
CY5 ulawulo lwangaphakathi

Isithinteli sokuphendula B

VIC/HEX Ufuzo lwe-methylated SFRP2
ROX Ufuzo lwe-methylated BMP3
FAM Ufuzo lwe-SDC2 lwemethylated
CY5 ulawulo lwangaphakathi

Ukutolikwa

Gene

Umjelo woMqondiso

Ct Ixabiso

Ukutolikwa

I-NDRG4

VIC (HEX)

ixabiso Ct≤38

I-NDRG4 ilungile

Ct ixabiso>38 okanye unde

I-NDRG4 embi

SEPT9

ROX

ixabiso Ct≤38

SEPT9 ilungile

Ct ixabiso>38 okanye unde

SEPT9 engalunganga

SFRP2

VIC (HEX)

ixabiso Ct≤38

SFRP2 positive

Ct ixabiso>38 okanye unde

SFRP2 negative

Iiparamitha zobuGcisa

Ugcino Ulwelo: ≤-18℃
Beka ubomi kwishelufa Iinyanga ezili-9
Uhlobo loMfanekiso Isampulu yesitulo
CV ≤5.0%
Ukuchaza ngokuthe ngqo Akukho kuphinda kusebenze kunye nomhlaza wesibindi, umhlaza wenyongo, umhlaza wamadlala kunye nomhlaza wemiphunga
Izixhobo Ezisebenzayo I-QuantStudio ®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

Ukuhamba komsebenzi

Ukukhutshwa kwe-reagent ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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