Iindidi ezingama-28 zoMngcipheko ophezulu weNtsholongwane yePapilloma yoMntu (16/18 Ukuchwetheza) i-Nucleic Acid

Iindidi ezingama-28 zoMngcipheko ophezulu weNtsholongwane kaBantu (16/18 Ukuchwetheza) I-Nucleic Acid eFakelwe umfanekiso

Inkcazelo emfutshane:

Le khithi ifanelekile kwi-in vitro yokubona umgangatho weentlobo ezingama-28 ze-human papilloma viruss (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) i-nucleic acid kumchamo wendoda / wesetyhini kunye neeseli ze-cervical exfoliated.I-HPV 16/18 inokuchwethezwa, iintlobo ezishiyekileyo azikwazi ukuchwethezwa ngokupheleleyo, zibonelela ngeendlela ezincedisayo zokuxilongwa kunye nonyango losulelo lwe-HPV.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-CC006A-28 Iintlobo zeNtsholongwane ePhakamileyo yePapilloma yoMntu (16/18 Ukuchwetheza) Ikhithi yokubona i-Nucleic Acid (Fluorescence PCR)

I-Epidemiology

Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kwindlela yokuzala yabasetyhini.Uphononongo lubonise ukuba usulelo oluzingisileyo lwe-HPV kunye nosulelo oluninzi ngomnye woonobangela abaphambili bomhlaza womlomo wesibeleko.Okwangoku, unyango olusebenzayo oluvunyiweyo lusanqongophele umhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nothintelo losulelo lomlomo wesibeleko olubangelwa yi-HPV ngundoqo ekuthinteleni umhlaza womlomo wesibeleko.Kubaluleke kakhulu ukuseka uvavanyo olulula, olucacileyo kunye nolukhawulezayo lwe-etiology yokuxilongwa kwekliniki kunye nonyango lomhlaza womlomo wesibeleko.

Umjelo

Umxube wokusabela	Umjelo	Uhlobo
PCR-Mix1	FAM	18
	VIC(HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Ulawulo lwangaphakathi
I-PCR-Mix2	FAM	6, 11, 54, 83
	VIC(HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino	Ulwelo: ≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	iseli yomlomo wesibeleko exfoliated
Ct	≤28
CV	≤5.0%
LoD	300Iikopi/mL
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Iinkqubo zePCR zeLightCycler®480 LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1.

Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ukhetho lwesi-2.

Ii-reagents zokutsalwa ezicetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye iKhiti yokuCoca(YDP315) yi-Tiangen Biotech (Beijing) Co.,Ltd.

Ngaphambili: I-Prostate Specific Antigen(PSA)

Okulandelayo: I-Papillomavirus yabantu (iintlobo ezingama-28) I-Genotyping

Bhala umyalezo wakho apha kwaye uwuthumele kuthi