Iindidi ze-15 ze-Papillomavirus yoMntu oMngcipheko ophezulu we-E6/E7 Gene mRNA

Inkcazelo emfutshane:

Le khithi ijolise ekuboneni umgangatho we-15 ephezulu yomngcipheko we-human papillomavirus (HPV) E6 / E7 gene mRNA amanqanaba okuzibonakalisa kwiiseli ezikhutshiweyo zomlomo wesibeleko.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-CC005A-15 Iintlobo zePapillomavirus yoMntu esemngciphekweni ophezulu E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)

I-Epidemiology

Umhlaza womlomo wesibeleko lolona hlobo luxhaphakileyo lomhlaza wabasetyhini kwihlabathi jikelele, kwaye ukwenzeka kwawo kunxulumene ngokusondeleyo ne-human papillomaviruses (HPV), kodwa liqaqobana elincinane losulelo lwe-HPV elinokuthi libe ngumhlaza.Umngcipheko ophezulu we-HPV wosulela iiseli ze-epithelial zomlomo wesibeleko kwaye uvelise ii-oncoprotein ezimbini, i-E6 kunye ne-E7.Le proteni inokuchaphazela iindidi zeeprotheni zeselula (ezifana ne-tumor suppressor proteins pRB kunye ne-p53), ukwandisa umjikelo weseli, ichaphazela i-DNA synthesis kunye nokuzinza kwe-genome, kwaye iphazamise iimpendulo ze-antiviral kunye ne-antitumor immune.

Umjelo

Umjelo Icandelo I-Genotype ivavanyiwe
FAM I-HPV Reaction Buffer 1 HPV16,31,33,35,51,52,58
VIC/HEX Ijini ye-β-actin yomntu
FAM I-HPV Reaction Buffer 2 HPV 18,39,45,53,56,59,66,68
VIC/HEX Ufuzo lwe-INS lomntu

Iiparamitha zobuGcisa

Ugcino Ulwelo: ≤-18℃
Beka ubomi kwishelufa Iinyanga ezili-9
Uhlobo loMfanekiso iseli yomlomo wesibeleko exfoliated
Ct ≤38
CV ≤5.0%
LoD Iikopi ezingama-500/mL
Izixhobo Ezisebenzayo Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukukhutshwa kwe-reagent ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa. .Umthamo we-elution ocetyiswayo yi-50μL.Ukuba isampulu ayigatyiswanga ngokupheleleyo, yibuyisele kwinyathelo lesi-4 lokwetyisa kwakhona.Uze uvavanye ngokwemiyalelo yokusetyenziswa.

I-reagent yokutsalwa ecetyiswayo: I-RNAprep iPure Animal Tissue Total RNA Extraction Kit (DP431).Ukutsalwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa ngokungqongqo (Kwinqanaba lesi-5, kabini ukuxinana kwesisombululo sokusebenza se-DNaseI, oko kukuthi, thatha i-20μL ye-RNase-Free DNaseI (1500U) isisombululo sesitokhwe kwi-RNase-Free centrifuge tube, yongeza i-60μL ye-RDD buffer, kwaye udibanise ngobunono).Umthamo we-elution ocetyiswayo yi-60μL.Ukuba isampulu ayigatyiswanga ngokupheleleyo, yibuyisele kwinyathelo lesi-5 lokwetyisa kwakhona.Uze uvavanye ngokwemiyalelo yokusetyenziswa.


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