I-Enterococcus enganyangekiyo kuVancomycin kunye neGene enganyangekiyo ngamachiza
Igama lemveliso
I-HWTS-OT090-Vancomycin-resistant Enterococcus kunye ne-Gene Restant-resistant Kit (i-PCR ye-Fluorescence)
I-Epidemiology
Ukumelana neChiza kukwabizwa ngokuba kukunganyangeki kwechiza, kubhekiselele kuxhathiso lwebhaktheriya kwisenzo samachiza okulwa neentsholongwane.Nje ukuba uxhathiso lwamachiza lwenzekile, umphumo wechemotherapy wamachiza uya kuncitshiswa kakhulu.Ukumelana neChiza kwahlulwe kuxhathiso lwangaphakathi kunye noxhathiso olufunyenweyo.Ukuchasana kwangaphakathi kuchongwa yi-bacterial chromosomal genes, edluliselwe kwisizukulwana ukuya kwisizukulwana, kwaye ayiyi kutshintsha.Ukuxhathisa okufunyenweyo kungenxa yokuba emva kokunxibelelana namayeza okubulala iintsholongwane, iibhaktheriya zitshintsha iindlela zazo zemetabolism ukuze zingabulawa ngamayeza okubulala iintsholongwane.
Iijini zokuxhathisa ivancomycin iVanA kunye neVanB zifunyenwe ukunganyangeki ngamachiza, apho iVanA ibonisa amanqanaba aphezulu okuxhathisa ivancomycin kunye neteicoplanin, iVanB ibonisa amanqanaba ahlukeneyo okuxhathisa kwivancomycin, kwaye inovelwano kwiteicoplanin.IVancomycin isoloko isetyenziswa ezonyango ukunyanga usulelo lwentsholongwane yeGram-positive, kodwa ngenxa yokuvela kwe vancomycin-resistant enterococci (VRE), ngakumbi i-enterococcus faecalis kunye ne-enterococcus faecium, ebalelwa ngaphezulu kwe-90%, izise imingeni emikhulu kunyango lwezonyango. .Okwangoku, akukho ichiza elithile le-antibacterial kunyango lwe-VRE.Ngaphezu koko, i-VRE inokudlulisela imizila yokumelana neziyobisi kwezinye ii-enterococci okanye ezinye iibhaktheriya ezine-Gram-positive.
Umjelo
| FAM | I-Vancomycin-resistant enterococci (VRE): Enterococcus faecalis kunye ne-Enterococcus faecium |
| VIC/HEX | Ulawulo lwangaphakathi |
| CY5 | vancomycin ukuxhathisa ijini iVanB |
| ROX | vancomycin resistance gene VanA |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | ikhohlela, igazi, umchamo okanye iikholoni ezimsulwa |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye nezinye izifo zokuphefumla ezifana klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus aeruginosa, jumophilus influenza. escherichia coli, I-pseudomonas fluorescens, i-candida albicans, i-chlamydia pneumoniae, i-adenovirus yokuphefumula, okanye iisampulu ziqulethe ezinye iintlobo ze-CTX, i-mecA, i-SME, iSampulu ze-SHV kunye ne-TEM. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .
