I-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus Nucleic Acid
Igama lemveliso
I-HWTS-OT062-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Staphylococcus aureus yenye yeebhaktheriya ezibalulekileyo ze-pathogenic zosulelo lwe-nosocomial.I-Staphylococcus aureus (SA) yeye-staphylococcus kwaye imele ibhaktheriya ye-Gram-positive, enokuvelisa iindidi ze-toxins kunye nee-enzymes ezihlaselayo.Iibhaktheriya zineempawu zokusabalalisa ngokubanzi, i-pathogenicity enamandla kunye nesantya esiphezulu sokumelana.I-nuclease gene ye-Thermostable (i-nuc) yimfuza egcinwe kakhulu ye-staphylococcus aureus.Kwiminyaka yakutshanje, ngenxa yokusetyenziswa kakhulu kwamahomoni kunye namalungiselelo omzimba kunye nokusetyenziswa kakubi kwe-antibiotics ebanzi, izifo ze-nosocomial ezibangelwa yi-Methicillin-resistant Staphylococcus aureus (MRSA) kwi-Staphylococcus ziye zanda.Umyinge welizwe jikelele wokuchongwa kwe-MRSA ibingama-30.2% ngo-2019 eTshayina.I-MRSA ihlukaniswe yaba yi-MRSA (HA-MRSA) enxulumene noluntu, i-MRSA (CA-MRSA), kunye ne-MRSA enxulumene nemfuyo (LA-MRSA).I-CA-MRSA, i-HA-MRSA, i-LA-MRSA inomahluko omkhulu kwi-microbiology, ukuxhathisa ibhaktheriya (umzekelo, i-HA-MRSA ibonisa ukuxhathisa kwamachiza amaninzi kune-CA-MRSA) kunye neempawu zeklinikhi (umz. indawo yokusuleleka).Ngokwale mpawu, i-CA-MRSA kunye ne-HA-MRSA inokuhlukaniswa.Nangona kunjalo, ukungafani phakathi kwe-CA-MRSA kunye ne-HA-MRSA kuyancipha ngenxa yokuhamba rhoqo kwabantu phakathi kwezibhedlele kunye noluntu.I-MRSA ixhatshazwa ngamachiza amaninzi, ayixhathisi kuphela kwi-antibiotics ye-β-lactam, kodwa kunye ne-aminoglycosides, i-macrolides, i-tetracyclines kunye ne-quinolones ukuya kumaqondo ahlukeneyo.Kukho iiyantlukwano ezinkulu zengingqi kumazinga okumelana namachiza kunye neentsingiselo ezahlukeneyo.
I-Methicillin yokumelana ne-mecA gene idlala indima eqinisekileyo kuxhathiso lwe-staphylococcal.I-gene iqhutyelwa kwi-mobile genetic element (SCCmec), efaka i-penicillin-binding protein 2a (PBP2a) kwaye inobudlelwane obuphantsi kwi-antibiotics ye-β-lactam, ukwenzela ukuba iziyobisi ezichasayo azikwazi ukuthintela ukuhlanganiswa kwe-cell wall peptidoglycan layer, okukhokelela kuxhathiso kumachiza.
Umjelo
| FAM | Uhlobo lofuzo lwe-methicillin-resistant mecA |
| CY5 | staphylococcus aureus nuc gene |
| VIC/HEX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isikhohlela, ulusu kunye neesampulu zosulelo lwezicubu ezithambileyo, kunye neesampulu zegazi elipheleleyo |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL |
| Ukuchaza ngokuthe ngqo | Akukho ukuhlangana kwakhona kunye nezinye izifo zokuphefumla ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsinemantococcus ye-pneumonia, i-klebsinemantobamiraenteritis, i-klebsimanobaillanicoccus, i-klebsinemianiella i-cter cloacae, i-streptococcus pneumoniae , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
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