Ukwahluka kwe-SARS-CoV-2
Igama lemveliso
I-HWTS-RT072A-SARS-CoV-2 Izixhobo Zokuchongwa kweeNguqulelo (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Inoveli coronavirus (SARS-CoV-2) isasazeke kwinqanaba elikhulu kwihlabathi liphela.Kwinkqubo yokusasaza, utshintsho olutsha luhlala lusenzeka, lukhokelela kutshintsho olutsha.Le mveliso isetyenziselwa ukufumanisa okuncedisayo kunye nokwahlulwa kwamatyala anxulumene nosulelo emva kokusasazeka okukhulu kweAlpha, Beta, Gamma, Delta kunye ne-Omicron yokuguquguquka kweengxaki ukusukela ngoDisemba ka-2020.
Umjelo
| FAM | N501Y, HV69-70del |
| CY5 | 211-212del, K417N |
| VIC(HEX) | E484K, Ulawulo lwangaphakathi |
| ROX | I-P681H, L452R |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-nasopharyngeal swabs, i-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1000Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda-phinda kusebenze kunye ne-coronavirus yabantu i-SARS-CoV kunye nezinye iipathogens eziqhelekileyo. |
| Izixhobo Ezisebenzayo: | IQuantStudio™5 Iinkqubo zePCR zeXesha langempela I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.
