SARS-CoV-2 umkhuhlane A umkhuhlane B Nucleic Acid Idityanisiwe
Igama lemveliso
HWTS-RT060A-SARS-CoV-2 umkhuhlane A umkhuhlane B iNucleic Acid eDityanisiweyo yeKhithi yokubona (i-Fluorescence PCR)
I-Epidemiology
I-Corona Virus Disease 2019 (COVID-19) ibangelwa yi-SARS-CoV-2 eyeye-β Coronavirus yodidi.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye isihlwele sichaphazeleka ngokubanzi.Okwangoku, abaguli abosulelwe yi-SARS-CoV-2 ngoyena mthombo wosulelo, kwaye izigulana ezingenazimpawu zinokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7.Eyona nto yayibonakaliswa ngumkhuhlane, ukukhohlela okomileyo kunye nokudinwa.Izigulana ezimbalwa zineempawu ezifana nokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye norhudo.
Umkhuhlane lusulelo olubukhali lokuphefumla olubangelwa yintsholongwane yomkhuhlane.Iyosulela kakhulu kwaye isasazeka kakhulu ngokukhohlela kunye nokuthimla.Idla ngokuqhambuka entwasahlobo nasebusika.Zintathu iindidi zoMkhuhlane, uMkhuhlane A (IFV A), uMkhuhlane B (IFV B) kunye noMkhuhlane C (IFV C), zombini ziphuma kusapho lwe-ortomyxovirus.I-Influenza A kunye ne-B, ene-single-stranded, i-segmental RNA viruses, yizona zizathu eziphambili zezifo zabantu.I-Influenza A sisifo esosulelayo esinamandla sokuphefumula, kuquka i-H1N1, i-H3N2 kunye nezinye ii-subtypes, kulula ukuyitshintsha.Uqhambuko lwehlabathi jikelele, "i-shift" ibhekisa kuguquko lomkhuhlane A, olukhokelela kuhlobo olutsha lwentsholongwane.Umkhuhlane B wohlulwe ngokwemizila emibini: Yamagata kunye neVictoria.Umkhuhlane B une-antigenic drift kuphela, kwaye bayakuphepha ukubekw' esweni kunye nokupheliswa kwenkqubo yomzimba yokuzikhusela ngoguqulo.Kodwa iintsholongwane zomkhuhlane B zivela kancinane kunomkhuhlane womntu A, wona obangela usulelo lokuphefumla kunye nobhubhane ebantwini.
Umjelo
FAM | I-SARS-CoV-2 |
ROX | IFV B |
CY5 | IFV A |
VIC(HEX) | Ijene yoLawulo lwangaphakathi |
Iiparamitha zobuGcisa
Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
I-Lyophilization: ≤30℃ ebumnyameni | |
Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 |
I-Lyophilization: iinyanga ezili-12 | |
Uhlobo loMfanekiso | I-Nasopharyngeal swabs, i-Oropharyngeal swabs |
Ct | ≤38 |
CV | ≤5.0% |
LoD | Iikopi ezingama-300/mL |
Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lomnqamlezo zibonise ukuba ikhithi iyahambelana ne-coronavirus yabantu i-SARSr- CoV, i-MERSr-CoV, i-HcoV-OC43, i-HcoV-229E, i-HcoV-HKU1, i-HCoV-NL63, intsholongwane yokuphefumla ye-syncytial virus A kunye ne-B, i-parainfluenza virus 1, 2 kunye 3, i-rhinovirusA, B kunye no-C, i-adenovirus 1, 2, 3, 4, 5, 7 kunye ne-55, i-metapneumovirus yabantu, i-enterovirus A, B, C kunye no-D, i-human cytoplasmic pulmonary virus, i-virus ye-EB, intsholongwane yemasisi I-cytomegalovirus yabantu, i-rotavirus, norovirus, intsholongwane kaqwilikana, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella inyumoniya, Mycobacterium Asperm, Casperates, Casperates, Casperatalus tuber bekungekho reaction phakathi kwePneumocystis yersini kunye neCryptococcus neoformans. |
Izixhobo Ezisebenzayo: | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.