Iintlobo ezintandathu zentsholongwane yokuphefumla

Iintlobo ezintandathu zeentsholongwane zokuphefumla Umfanekiso obonakalayo

Inkcazelo emfutshane:

Le khithi ingasetyenziselwa ukubona ngokufanelekileyo i-nucleic acid ye-SARS-CoV-2, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B, i-adenovirus, i-mycoplasma pneumoniae kunye nentsholongwane yokuphefumla ye-syncytial in vitro.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT058A/B/C/Z-Ikhithi yexesha lokwenyani le-fluorescent RT-PCR yokubona iintlobo ezintandathu zentsholongwane yokuphefumla

Isatifikethi

I-Epidemiology

Isifo se-Corona Virus 2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwinyumoniya ebangelwa lusulelo lwe-SARS-CoV-2.I-SARS-CoV-2 yi-coronavirus yodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi.Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-SARS-CoV-2, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7.Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa.Izigulana ezimbalwa zazixinana ngeempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye norhudo.

Umkhuhlane, owaziwa ngokuba "ngumkhuhlane", sisifo esosulelayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane.Iyosulela kakhulu.Isasazwa ikakhulu ngokukhohlela kunye nokuthimla.Idla ngokuqhambuka entwasahlobo nasebusika.Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A, IFV A, umkhuhlane B, IFV B, kunye noMkhuhlane C, IFV C iindidi ezintathu, zonke zezentsholongwane encangathi, zibangela isifo somntu ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yintsholongwane enye, intsholongwane ye-RNA.Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele."Shift" ibhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kwentsholongwane entsha "subtype".Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini, iYamagata kunye neVictoria.Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye iyayiphepha iliso lenkqubo yomzimba yokuzikhusela kunye nokuphelisa ngokuguqulwa kwayo.Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu.Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.

I-Adenovirus (AdV) yeye-mammalian adenovirus, eyintsholongwane ephindwe kabini ye-DNA ngaphandle kwemvulophu.Ubuncinci i-90 genotypes ifunyenwe, enokwahlulwa ibe yi-AG 7 subgenera.Usulelo lwe-AdV lunokubangela izifo ezahlukahlukeneyo, kuquka inyumoniya, ibronchitis, icystitis, i-eye conjunctivitis, izifo zesisu kunye ne-encephalitis.I-Adenovirus pneumonia yenye yeentlobo ezinzima kakhulu zenyumoniya ezifunyenwe kuluntu kubantwana, zibalelwa malunga ne-4% -10% ye-pneumonia efunyenwe kuluntu.

I-Mycoplasma pneumoniae (MP) luhlobo lweyona microorganism encinci yeprokaryotic, ephakathi kwebhaktheriya kunye nentsholongwane, kunye nesakhiwo seseli kodwa akukho ludonga lweseli.I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla yabantu, ngakumbi ebantwaneni nakubantu abancinci.Inokubangela inyumoniya ye-mycoplasma yabantu, usulelo lwendlela yokuphefumla yabantwana kunye nenyumoniya engaqhelekanga.Ukubonakaliswa kweklinikhi kuyahluka, uninzi lwazo lukhohlokhohlo olunzima, umkhuhlane, ukugodola, intloko ebuhlungu, umqala obuhlungu.Ukosuleleka kwendlela yokuphefumula ephezulu kunye nenyumoniya yebronchial zezona zixhaphakileyo.Ezinye izigulana zinokukhula ukusuka kusulelo lwendlela yokuphefumla ephezulu ukuya kwinyumoniya ebukhali, uxinzelelo olukhulu lokuphefumla kunye nokufa kungenzeka.

Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae.Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana.Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali (ekubhekiswa kuyo njenge-bronchiolitis) kunye nenyumoniya, ezinxulumene nesifo sombefu ebantwaneni.Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia.Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl.

Umjelo

Igama letshaneli	I-R6 Isithinteli sokuphendula A	I-R6 Isithinteli sokuphendula B
FAM	I-SARS-CoV-2	I-HAdV
VIC/HEX	Ulawulo lwangaphakathi	Ulawulo lwangaphakathi
CY5	IFV A	MP
ROX	IFV B	RSV

Iiparamitha zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Beka ubomi kwishelufa	Ulwelo: iinyanga ezili-9;I-Lyophilized: iinyanga ezili-12
Uhlobo loMfanekiso	Igazi elipheleleyo, iPlasma, iSerum
Ct	≤38
CV	≤5.0％
LoD	300Iikopi/mL
Ukuchaza ngokuthe ngqo	Iziphumo ze-cross-reactivity zibonise ukuba akukho mpendulo phakathi kwekiti kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, uhlobo lwentsholongwane ye-parainfluenza 1, 2, 3, I-rhinovirus A, B, C, i-chlamydia pneumoniae, i-metapneumovirus yabantu, i-enterovirus A, B, C, D, intsholongwane ye-pulmonary yabantu, intsholongwane ye-epstein-barr, intsholongwane yemasisi, intsholongwane ye-cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kunye ne-cryptococcus esanda kuzalwa kunye ne-nucleic acid yabantu.
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike SLAN-96P Iinkqubo zePCR zeXesha langempela Iinkqubo zePCR ze-ABI 7500 ABI 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela Iinkqubo zePCR zeLightCycler®480 LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo ye-PCR yexesha lokwenene, i-BioRad CFX Opus 96 Real-Time PCR System