Ikhithi ye-Fluorescent ye-RT-PCR yexesha lokwenyani yokubona i-SARS-CoV-2
Igama lemveliso
Ikhithi ye-HWTS-RT057A-Ixesha lokwenyani le-fluorescent RT-PCR yokubona i-SARS-CoV-2
Ikhithi ye-HWTS-RT057F-Mkhenkce-yomileyo yexesha lokwenyani ye-RT-PCR yokubhaqa i-SARS-CoV-2 -Ipakethe
Isatifikethi
CE
I-Epidemiology
Inoveli coronavirus (SARS-CoV-2) isasazeke kwinqanaba elikhulu kwihlabathi liphela.Kwinkqubo yokusasaza, utshintsho olutsha luhlala lusenzeka, lukhokelela kutshintsho olutsha.Le mveliso isetyenziselwa ukufumanisa okuncedisayo kunye nokwahlulwa kwamatyala anxulumene nosulelo emva kokusasazeka okukhulu kweAlpha, Beta, Gamma, Delta kunye ne-Omicron yokuguquguquka kweengxaki ukusukela ngoDisemba ka-2020.
Umjelo
| FAM | Uhlobo lwe-2019-nCoV ORF1ab |
| CY5 | 2019-nCoV N yejini |
| VIC(HEX) | ijini yesalathiso yangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
| I-Lyophilized: ≤30℃ ebumnyameni | |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 |
| I-Lyophilized: iinyanga ezili-12 | |
| Uhlobo loMfanekiso | i-nasopharyngeal swabs, i-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 300Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda-phinda kusebenze kunye ne-coronavirus yabantu i-SARS-CoV kunye nezinye iipathogens eziqhelekileyo. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ukutsalwa kwe-Acid yeNucleic okanye iKit yokuCoca(iNdlela yeMagnetic Beads)(HWTS-3001, HWTS-3004-32, HWTS-3004-48) esuka kwiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ukhetho lwesi-2.
Isixhobo sokukhutshwa esicetyiswayo: QIAamp Viral RNA Mini Kit (52904), Viral RNA Extraction Kit (YDP315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd.
