I-Mycobacterium Tuberculosis Rifampicin Resistance
Igama lemveliso
HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Kit (Fluorescence PCR)
I-Epidemiology
I-Rifampicin isetyenziswe ngokubanzi kunyango lwezigulane ze-pulmonary tuberculosis ukususela ekupheleni kwe-1970s, kwaye inesiphumo esibalulekileyo.Kuye kwaba yinto yokuqala yokukhetha ukunciphisa i-chemotherapy yezigulane ze-pulmonary tuberculosis.Ukumelana ne-Rifampicin kubangwa ikakhulu kukuguqulwa kwejini ye-rpoB.Nangona amachiza amatsha okulwa nesifo sephepha ehlala ephuma, kunye nokusebenza kwezonyango kwizigulana zesifo sephepha semiphunga nako kuqhubekile ukuphucuka, kusekho ukunqongophala kweziyobisi ezichasene nesifo sephepha, kwaye isenzeko sokusetyenziswa kweziyobisi ngokungekho ngqiqweni ekliniki siphezulu.Ngokucacileyo, isifo sephepha seMycobacterium kwizigulane ezine-pulmonary tuberculosis ayikwazi ukubulawa ngokupheleleyo ngexesha elifanelekileyo, ekugqibeleni likhokelela kwiinqanaba ezahlukeneyo zokunganyangeki kweziyobisi emzimbeni wesigulane, kwandisa ixesha lesi sifo, kwaye kwandisa umngcipheko wokufa kwesigulane.Le khithi ilungele uxilongo oluncedisayo losulelo lwesifo sephepha seMycobacterium kunye nokufunyanwa kwejini yokuxhathisa i-rifampicin, eluncedo ukuqonda ukuxhathisa kumachiza esifo sephepha se-mycobacterium esosulelwe zizigulane, kunye nokubonelela ngeendlela ezincedisayo kwisikhokelo samayeza ezonyango.
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Isikhohlela |
| CV | ≤5.0% |
| LoD | uhlobo lwasendle oluxhathisa i-rifampicin: 2x103ibhaktheriya/mL i-homozygous mutant: 2x103ibhaktheriya/mL |
| Ukuchaza ngokuthe ngqo | Le khithi ayinayo i-cross-reaction kunye ne-genome yomntu, enye i-mycobacteria engeyiyo-tuberculous, kunye ne-pneumonia pathogens.Ibhaqa iziza zotshintsho lwezinye iindlela zokumelana namachiza zohlobo lwasendle lwe-mycobacterium tuberculosis ezifana ne-katG 315G>C\A, InhA-15C> T, iziphumo zovavanyo zibonisa ukunganyangeki kwi-rifampicin, nto leyo ethetha ukuba akukho mpendulo idibanayo. |
| Izixhobo Ezisebenzayo: | SLAN-96P Iinkqubo zePCR zeXesha langempela |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.
