I-Mycobacterium Tuberculosis I-Isoniazid Resistance Detection Kit(Fluorescence PCR)
Igama lemveliso
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Isoniazid, ichiza eliphambili lokulwa isifo sephepha elaziswa ngo-1952, lelinye lawona machiza asebenzayo kunyango oludibeneyo lwesifo sephepha esisebenzayo kunye nechiza elinye lesifo sephepha esafukamileyo.
I-KatG yeyona jini iphambili yokufaka ikhowudi ye-catalase-peroxidase kwaye i-katG yokuguqulwa kofuzo inokukhuthaza ukuhlanganiswa kodonga lweseli ye-mycolic acid, yenze ibhaktiriya imelane ne-isoniazid.Ukubonakaliswa kwe-KatG kuhambelana kakubi kunye neenguqu kwi-INH-MIC, kunye nokuncipha kwe-2-fold in katG yokubonisa kubangela ukunyuka okuncinci kwe-2 kwi-MIC.Omnye unobangela wokunganyangeki kwe-isoniazid kwi-mycobacterium tuberculosis kwenzeka xa ukufakwa kwesiseko, ukucima okanye ukuguquka kwenzeka kwi-InhA gene locus ye-mycobacterium tuberculosis.
Umjelo
| ROX | inhA (-15C>T) indawo· |
| CY5 | katG (315G>C) indawo |
| VIC (HEX) | IS6110 |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isikhohlela |
| CV | ≤5.0% |
| LoD | 1 × 103ibhaktheriya/mL |
| Ukuchaza ngokuthe ngqo | I-No-cross reactivity kunye nokuguqulwa kweendawo ezine zokumelana neziyobisi (511, 516, 526 kunye ne-531) ye-rpoB gene ngaphandle koluhlu lokufumanisa ikiti yokufumanisa. Izixhobo Ezisebenzayo: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test yeSampuli yokuKhupha iReagent (HWTS-3005-8).
Ukhetho lwesi-2.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
