Middle East Respiratory Syndrome ICoronavirus Nucleic Acid
Igama lemveliso
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
I-Epidemiology
I-Middle East yokuphefumla isifo se-coronavirus (MERS-CoV), i-β-coronavirus ebangelaIsifo sokuphefumla ebantwini, yaqala ukuchongwa kwisigulane esineminyaka engama-60 ubudala saseSaudi Arabia esifayo ngomhla wama-24 kaJulayi, 2012. Umboniso weklinikhi we-MERS-CoV usulelo lusuka kwi-asymptomatic state okanye iimpawu zokuphefumula ezithambileyo ukuya kwisifo esibi kakhulu sokuphefumla nokufa.
Umjelo
FAM | Intsholongwane ye-MERS RNA |
VIC(HEX) | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
Ugcino | ≤-18℃ ebumnyameni |
Beka ubomi kwishelufa | Iinyanga ezili-9 |
Uhlobo loMfanekiso | Iiswabs ze-nasopharyngeal ezisanda kuqokelela |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 Iikopi/mL |
Ukuchaza ngokuthe ngqo | Akukho kuphinda kusebenze kunye ne-coronavirus yabantu i-SARSr-CoV kunye nezinye iipathogens eziqhelekileyo. |
Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-Reagent yokutsalwa ecetyiswayo: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction okanye Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.
Ukhetho lwesi-2.
I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).