Middle East Respiratory Syndrome ICoronavirus Nucleic Acid

Inkcazelo emfutshane:

Ikhithi isetyenziselwa ukufumanisa umgangatho we-MERS coronavirus nucleic acid kwi-nasopharyngeal swabs kunye ne-Middle East Respiratory Syndrome (MERS) coronavirus.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

I-Epidemiology

I-Middle East yokuphefumla isifo se-coronavirus (MERS-CoV), i-β-coronavirus ebangelaIsifo sokuphefumla ebantwini, yaqala ukuchongwa kwisigulane esineminyaka engama-60 ubudala saseSaudi Arabia esifayo ngomhla wama-24 kaJulayi, 2012. Umboniso weklinikhi we-MERS-CoV usulelo lusuka kwi-asymptomatic state okanye iimpawu zokuphefumula ezithambileyo ukuya kwisifo esibi kakhulu sokuphefumla nokufa.

Umjelo

FAM Intsholongwane ye-MERS RNA
VIC(HEX)

Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino

≤-18℃ ebumnyameni

Beka ubomi kwishelufa

Iinyanga ezili-9

Uhlobo loMfanekiso

Iiswabs ze-nasopharyngeal ezisanda kuqokelela

CV

≤5.0%

Ct

≤38

LoD

1000 Iikopi/mL

Ukuchaza ngokuthe ngqo

Akukho kuphinda kusebenze kunye ne-coronavirus yabantu i-SARSr-CoV kunye nezinye iipathogens eziqhelekileyo.

Izixhobo Ezisebenzayo:

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1.

I-Reagent yokutsalwa ecetyiswayo: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction okanye Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.

Ukhetho lwesi-2.

I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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