Umkhuhlane B Virus Nucleic Acid
Igama lemveliso
HWTS-RT127A-Influenza B Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
I-HWTS-RT128A-Isixhobo sokuQinisekisa soMkhuhlane B seNtsholongwane yeNucleic Nucleic Acid (Enzymatic Probe Isothermal Amplification)
Isatifikethi
CE
I-Epidemiology
Intsholongwane ye-Influenza, uhlobo olumele lwe-Orthomyxoviridae, yintsholongwane esongela kakhulu impilo yabantu kwaye inokosulela abantu abaninzi.Ubhubhani womkhuhlane wexesha lonyaka wosulela abantu abamalunga nezigidi ezingama-600 kwihlabathi liphela kwaye ubangela ukufa kwabantu abangama-250,000 ukuya kuma-500,000 minyaka le, apho intsholongwane yomkhuhlane u-B sesinye sezizathu eziphambili.[1].Intsholongwane yoMkhuhlane B, ekwaziwa ngokuba yi-IVB, yiRNA enemisonto enye ene-negative.Ngokulandelelana kwe-nucleotide yendawo ye-antigenic ye-HA1, inokwahlulwa ibe yimigca emibini emikhulu, i-strain emele i-B / Yamagata / 16/88 kunye ne-B / Victoria / 2/87 (5)[2].Intsholongwane yoMkhuhlane B iqhelekile ukuba neempawu ezicacileyo zomamkeli.Kufunyaniswe ukuba i-IVB inokosulela kuphela abantu kunye netywina, kwaye ayibangeli ubhubhani wehlabathi jikelele, kodwa inokubangela ubhubhani wexesha lonyaka.[3].Intsholongwane ye-Influenza B inokudluliselwa ngeendlela ezahlukeneyo ezifana nomgudu wokugaya, umgudu wokuphefumla, umonakalo wolusu kunye ne-conjunctiva.Iimpawu ikakhulu ngumkhuhlane ophezulu, ukhohlokhohlo, impumlo evuzayo, i-myalgia, njl njl. Uninzi lwazo lukhatshwa yinyumoniya ebukhali, ukuhlaselwa yintliziyo.Kwiimeko ezinzima, ukungaphumeleli kwentliziyo, izintso kunye namanye amalungu kukhokelela ekufeni, kwaye izinga lokufa liphezulu kakhulu[4].Ngoko ke, kukho imfuneko engxamisekileyo yendlela elula, echanekileyo nekhawulezayo yokufumanisa intsholongwane yomkhuhlane B, enokunika isikhokelo kumayeza ekliniki kunye nokuxilongwa.
Umjelo
FAM | IVB nucleic acid |
ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
Ugcino | Ulwelo: ≤-18℃ ebumnyameni I-Lyophilization: ≤30℃ ebumnyameni |
Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 I-Lyophilization: iinyanga ezili-12 |
Uhlobo loMfanekiso | Iisampuli ze-Nasopharyngeal swab Iisampuli ze-Oropharyngeal swab |
CV | ≤10.0% |
Tt | ≤40 |
LoD | 1 Ikopi/µL |
Ukuchaza ngokuthe ngqo | akukho kuphinda-phinda kusebenze kunye ne-Influenza A, i-Staphylococcus aureus,Streptococcus (kubandakanya Streptococcus pneumoniae), Adenovirus, Mycoplasma pneumoniae, Respiratory Syncytial Virus, Mycobacterium tuberculosis, imasisi, Haemophilus influenzae, Rhinovirus, Coronavirus, Enteric Virus, swab yomntu osempilweni. |
Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela I-SLAN ® -96P Iinkqubo zePCR zeXesha langempela I-LightCycler® 480 inkqubo ye-PCR yeXesha langempela I-Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS1600) |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.