I-CYP2C9 yabantu kunye ne-VKORC1 Gene Polymorphism
Igama lemveliso
I-HWTS-GE014A-Human CYP2C9 kunye ne-VKORC1 Gene Polymorphism Detection Kit (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Warfarin yi-anticoagulant yomlomo esetyenziswa rhoqo kwiiklinikhi ngoku, ejoliswe ikakhulu kuthintelo kunye nonyango lwezifo ze-thromboembolic.Nangona kunjalo, i-warfarin inefestile yonyango elinganiselwe kwaye iyahluka kakhulu phakathi kweentlanga ezahlukeneyo kunye nabantu ngabanye.Izibalo zibonise ukuba umahluko wedosi ezinzileyo kubantu abahlukeneyo unokuba ngaphezu kwamaxesha angama-20.Ukopha okungalunganga kwenzeka kwi-15.2% yezigulane ezithatha i-warfarin ngonyaka, apho i-3.5% iphuhlisa ukopha okubulalayo.Uphononongo lwe-Pharmacogenomic lubonise ukuba i-polymorphism yofuzo ye-enzyme ekujoliswe kuyo i-VKORC1 kunye ne-enzyme ye-metabolic CYP2C9 ye-warfarin yinto ebalulekileyo echaphazela umahluko kwithamo le-warfarin.I-Warfarin yi-inhibitor ethile ye-vitamin K epoxide reductase (VKORC1), kwaye ngaloo ndlela inqanda i-clotting factor synthesis ebandakanya i-vitamin K kwaye ibonelela nge-anticoagulation.Inani elikhulu lezifundo libonise ukuba i-polymorphism yemfuza ye-VKORC1 umgqugquzeli yeyona nto ibalulekileyo echaphazela ugqatso kunye nokwahlukana komntu ngamnye kwidosi efunekayo ye-warfarin.I-Warfarin i-metabolized yi-CYP2C9, kwaye iinguqu zayo zinciphisa kakhulu imetabolism ye-warfarin.Abantu abasebenzisa i-warfarin banomngcipheko ophezulu (ophindwe kabini ukuya kathathu ngaphezulu) ukopha kwinqanaba lokuqala lokusetyenziswa.
Umjelo
| FAM | I-VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Igazi elitsha le-EDTA le-anticoagulated |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity kunye nolunye ulandelelwano oluhambelana kakhulu lwe-genome yomntu (i-CYP2C19 gene yomntu, i-RPN2 gene);ukuguqulwa kwe-CYP2C9*13 kunye ne-VKORC1 (3730G>A) ngaphandle koluhlu lokubona le khithi |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukukhutshwa kwe-reagent ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
