Human BRAF Gene V600E Ukuguqulwa
Igama lemveliso
I-HWTS-TM007-Human BRAF Gene V600E Ikhithi yokuFundisa iNguqu(Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Ngaphezulu kwe 30 iintlobo zotshintsho lwe-BRAF zifunyenwe, apho malunga ne-90% ibekwe kwi-exon 15, apho ukuguqulwa kwe-V600E kubonwa njengeyona nto ixhaphakileyo, oko kukuthi, i-thymine (T) kwindawo ye-1799 kwi-exon 15 iguqulelwe i-adenine (A), okubangelwa ukutshintshwa kwe-valine (V) kwindawo ye-600 nge-glutamic acid (E) kwimveliso yeprotheni.Iinguqu ze-BRAF zihlala zifumaneka kwiithumba ezinobungozi ezifana ne-melanoma, umhlaza we-colorectal, umhlaza we-thyroid kunye nomhlaza wemiphunga.Ukuqonda uguqulo lwejini ye-BRAF ibe yimfuneko yokuhlola i-EGFR-TKIs kunye ne-BRAF gene mutation-targeted drug kunyango lwechiza olujoliswe eklinikhi kwizigulana ezinokuthi zixhamle.
Umjelo
| FAM | Ukuguqulwa kweV600E, ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | iparafini-embedded iisampulu ze-pathological tissue |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Sebenzisa iikhithi ukukhangela ulawulo lomgangatho we-LoD oluhambelanayo.a) phantsi kwe-3ng/μL imvelaphi yohlobo lwasendle, i-1% yesantya soguqulo inokubonwa kwi-reaction buffer ngokuzinzileyo;b) ngaphantsi kwe-1% yezinga lokuguqulwa, ukuguqulwa kwe-1 × 103Iikopi / mL kwimvelaphi yohlobo lwasendle lwe-1 × 105Iikopi/mL zinokubonwa ngokuzinzileyo kwi-reaction buffer;c) I-IC Reaction Buffer inokubona owona mda uphantsi wolawulo lomgangatho we-SW3 wolawulo lwangaphakathi lwenkampani. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempelaI-Biosystems esetyenzisiweyo ye-7300 ye-Real-Time PCR Iinkqubo, i-QuantStudio® 5 Iinkqubo ze-PCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene |
Ukuhamba komsebenzi
Ii-reagents zokutsalwa ezicetyiswayo: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), iParaffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yiTiangen Biotech(Beijing) Co., Ltd.
