Herpes Simplex Virus Uhlobo 1/2,(HSV1/2) Nucleic Acid
Igama lemveliso
I-HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) ikiti yokubona i-nucleic acid (i-Fluorescence PCR)
I-Epidemiology
Izifo ezosulela ngokwabelana ngesondo (Sexually Transmitted Diseases) (STD) iseyenye yezoyikiso ezinkulu kukhuseleko lwempilo yoluntu jikelele.Izifo ezinjalo zingakhokelela ekukhulelweni, ukunikezelwa komntwana ngaphambi kwexesha, i-tumor kunye neengxaki ezinzulu ezahlukeneyo.Kukho iintlobo ezininzi ze-STD pathogens, kubandakanywa ibhaktheriya, iintsholongwane, i-chlamydia, i-mycoplasma kunye ne-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, kunye ne-Ureaplasma urealyticum.
I-herpes ye-Genital sisifo esixhaphakileyo sokwabelana ngesondo esibangelwa yi-HSV2, eyosulela kakhulu.Kwiminyaka yakutshanje, izehlo ze-herpes zangasese ziye zanda kakhulu, kwaye ngenxa yokwanda kweendlela zokuziphatha zesondo ezinobungozi, izinga lokufumanisa i-HSV1 kwi-herpes yangasese liye landa kwaye kwaxelwa ukuba liphezulu njenge-20% -30%.Usulelo lokuqala kunye nentsholongwane ye-herpes yangasese ihlala ithule ngaphandle kweempawu zekliniki ezicacileyo ngaphandle kwe-herpes yendawo kwi-mucosa okanye ulusu lwezigulane ezimbalwa.Ekubeni i-herpes ye-genital ibonakaliswe ngokuchithwa kwentsholongwane ubomi bonke kunye nokuthambekela ekubuyeleni kwakhona, kubalulekile ukukhangela i-pathogens ngokukhawuleza kwaye uthintele usulelo lwayo.
Umjelo
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo loMfanekiso | ukukhutshwa kwe-urethral, ukukhutshwa komlomo wesibeleko |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Iikopi/impendulo |
Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity kunye nezinye izifo ze-STD ezifana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, kunye ne-Ureaplasma urealyticum. |
Izixhobo Ezisebenzayo | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.