Herpes Simplex Virus Uhlobo 1/2,(HSV1/2) Nucleic Acid

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-Herpes Simplex Virus Type 1 (HSV1) kunye ne-Herpes Simplex Virus Type 2 (HSV2) ukunceda ukuxilonga kunye nokunyanga izigulane ezinosulelo lwe-HSV ezikrokrelekayo.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) ikiti yokubona i-nucleic acid (i-Fluorescence PCR)

I-Epidemiology

Izifo ezosulela ngokwabelana ngesondo (Sexually Transmitted Diseases) (STD) iseyenye yezoyikiso ezinkulu kukhuseleko lwempilo yoluntu jikelele.Izifo ezinjalo zingakhokelela ekukhulelweni, ukunikezelwa komntwana ngaphambi kwexesha, i-tumor kunye neengxaki ezinzulu ezahlukeneyo.Kukho iintlobo ezininzi ze-STD pathogens, kubandakanywa ibhaktheriya, iintsholongwane, i-chlamydia, i-mycoplasma kunye ne-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, kunye ne-Ureaplasma urealyticum.

I-herpes ye-Genital sisifo esixhaphakileyo sokwabelana ngesondo esibangelwa yi-HSV2, eyosulela kakhulu.Kwiminyaka yakutshanje, izehlo ze-herpes zangasese ziye zanda kakhulu, kwaye ngenxa yokwanda kweendlela zokuziphatha zesondo ezinobungozi, izinga lokufumanisa i-HSV1 kwi-herpes yangasese liye landa kwaye kwaxelwa ukuba liphezulu njenge-20% -30%.Usulelo lokuqala kunye nentsholongwane ye-herpes yangasese ihlala ithule ngaphandle kweempawu zekliniki ezicacileyo ngaphandle kwe-herpes yendawo kwi-mucosa okanye ulusu lwezigulane ezimbalwa.Ekubeni i-herpes ye-genital ibonakaliswe ngokuchithwa kwentsholongwane ubomi bonke kunye nokuthambekela ekubuyeleni kwakhona, kubalulekile ukukhangela i-pathogens ngokukhawuleza kwaye uthintele usulelo lwayo.

Umjelo

FAM HSV1
CY5 HSV2
VIC(HEX) Ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino Ulwelo: ≤-18℃ ebumnyameni
Beka ubomi kwishelufa Iinyanga ezili-12
Uhlobo loMfanekiso ukukhutshwa kwe-urethral, ​​ukukhutshwa komlomo wesibeleko
Ct ≤38
CV ≤5.0%
LoD 50 Iikopi/impendulo
Ukuchaza ngokuthe ngqo Akukho-cross-reactivity kunye nezinye izifo ze-STD ezifana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, kunye ne-Ureaplasma urealyticum.
Izixhobo Ezisebenzayo Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike.

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1.

I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ukhetho lwesi-2.

I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.


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